Molecular characterization and phylogenetic analysis of pseudorabies virus isolated from pigs in Ukraine

The article presents the results of a molecular genetic study two isolates Pseudorabies virus that were isolated from pigs in Ukraine. Bioinformatic analysis gE gene fragment Aujeszky's disease (Pseudorabies virus) was carried out order to determine phylogenetic relationships and homology nucleotide sequences. Fragments Aujeszky genome corresponding C-terminal region selected for sequencing further analysis. As result conducted studies, it demonstrated sequences analyzed samples differ each other by presence ACG insert tandem repeats region. Comparison studied with strains/isolates found Europe Asia, presented GenBank database, indicates such an is characteristic Min-A HNJZ strains (position 1487 gene) Asia. Analysis showed sequence sample No. 1 100% identical 89V87 00V72 Belgium. 3 99.13%. In clarify belonging particular genogroup (genetic cluster), dendrogram constructed. This demonstrates between virus. It belong cluster uniting European strains/isolates, are most genetically close 00V72.